Cardiovascular tissue banking in Croatia
نویسندگان
چکیده
Methods Cardiovascular tissue allografts (CVA) were retrieved from recipients of heart transplants (RHT) and multiorgan donors (MOD) which fulfill donor selection criteria. Processing of CVA was performed in CTB clean room (GMP Class B). Initial processing included surgical dissection, measurements, morphology and functional assessment and collection of quality control samples. CVA were incubated in antibiotic containing medium (vancomycin, lincomycin, polymyxin B sulfate) for 24-48 hours. Then CVA were cryopreserved in solution containing 10% of dimethylsulphoxyde and stored in vapor phase of liquid nitrogen. Cryopreserved tissues remained in the quarantine until all quality control results were collected.
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